Procedures

 

Central Line

Central line kit (single or triple lumen)     

Blue IV adapter caps (3 for triple lumen)

Suture 2-0 silk               Needle driver                 

Chlorhexidine scrub        Saline flushes               

SiteRite & sleeve           Mask, gown and gloves

 

Helpful Subclavian Hints:  Place patient in Trendelenburg (15-20 degrees) and turn their head away from the target vessel.  Identify the lateral margin of the posterior belly of the SCM muscles as it inserts into the clavicle.  Your needle insertion site is 1 cm inferior and 1 cm lateral to the inferior aspect of the clavicle at this point.   Be sure to anesthetize the periosteum with Lidocaine.  Advance the needle until it hits the clavicle, pass the needle under the clavicle (keeping it parallel to the patient’s back…don’t dive in, there’s lung under there!)  As you pass the needle point towards the suprasternal notch…and you should hit the vein.  Check to make sure the blood is dark and not red and pulsatile (arterial).  Insert the catheter 15 cm for a Right sided subclavian, and 17 cm for the Left side.  If you hit the Subclavian artery, remove the needle and hold pressure above and below the clavicle (pinch the clavicle). 

 

Helpful Internal Jugular Hints:  Place patient in Trendelenburg (15-20 degrees) and turn their head away from the target vessel.  Identify the triangle formed by the anterior and posterior bellies of the SCM muscle and the clavicle.  Your insertion site is near the apex of this triangle.  Use the Site Rite in obese patients or in patients you suspect may have clots in this area (ie. frequently instrumented patients).  On the Site Rite, the vein will be compressible, while the artery will not be.  Palpate the carotid artery in the triangle and retract it medially.  Insert the needle at a 45-degree angle to the skin into the triangle apex just lateral to the carotid pulsation, advancing toward the ipsilateral nipple.     Insert the catheter 15 cm for a Right IJ, and 17 cm for a Left IJ.  If you hit the Carotid, remove needle and hold pressure.

 

If IJ or subclavian call for STAT CXR to confirm placement and r/o PTX

 

Helpful Femoral Vein Line Hints:  Identify the pulsation of the femoral artery, pull it laterally.  Needle insertion site is just medial to the pulsation, 1 cm inferior to the inguinal ligament.  Insert the catheter to the hub.  This line should only be used temporarily (a few days) as it has the highest risk of infection.

 

Radial Arterial Line

Basic tray                     +/-Lidocaine                 

Chlorhexidine scrub        Arrow-ART line   Suture              

Needle driver                  Tape to tape down hand

Ask Nurse to “set up for an A-line”

 

Helpful Radial Art-Line Hints:  Be sure to place patient’s hand in a hyperextended position (may have to tape down their hand to achieve this).  Palpate the radial artery with 2 fingertips placed 2 cm apart.  Delineate a line b/n the fingertips, and insert the angiocatheter along this line at a 45 degree angle from the skin, into the radial artery.  Once you get a flash of blood in the needle hub, advance the whole apparatus until the artery is transfixed.  Then withdraw the needle and leave the cannula in place.  Attach to the transducer and be sure to suture the cannula down.

 

Femoral Arterial Line

Single lumen central line kit        Basic tray

Suture   Needle driver     Chlorhexidine

Ask Nurse to “set up for an A-line”

 

Helpful Femoral Art-Line Hints:  Identify the pulsation of the femoral artery.  This is your insertion site.  Attach to the transducer, and suture the site down.

 

Lumbar Puncture

LP kit, Betadine (do not use chlorhexidine!), Band aid, Extra CSF tubes

 

The minimal CSF volumes needed for the following tests:

Cell and differential                                 1.0 cc

Oligoclonal bands                                  5.0 cc

Glucose                                                0.3 cc

Mycobacteria/AFB                                 5.0 cc

Protein                                                  0.4 cc

Mycology cx/Crypto Ag                          0.5 cc

Bacteriology                                          0.5 cc  

Virology                                                0.5 cc

Immunology/Meningitis screen                1.0 cc  

VDRL                                                    0.5 cc

IgG Index (for multiple sclerosis)              0.5 cc  

Cytopath                                               3.0 cc

 

You can often combine several of these tests together in one tube: e.g. tube #1 and tube #4 (1 cc each) for cell count and diff; tube #2 (1 cc) for protein/glucose; tube #3 (8 cc) for all of the microbiology which will later be distributed among the labs, except for VDRL which needs a separate tube.  Always remember to collect 1-2 extra “Did you” tubes for second-thought tests.  Labs can be ordered via ordernet or on a pager path rec (easier)

 

Helpful Lumbar Puncture Hints:  Position the patient either sitting on the edge of the bed leaning over a table, or lying on their side in a tight fetal position.  Palpate the superior edge of the iliac crests and create a line b/n it and the L4-L5 area.  After prep and local anesthesia, insert the needle deeper, anesthetizing the periosteum.  Insert the spinal needle between the spinous processes, angled towards the umbilicus.  Withdraw the stylet periodically, and you should get spinal fluid (generally this occurs after you feel a “pop”…the entry of the needle into the dura)

 

Paracentesis

Caldwell needle              Basic tray                                 Lidocaine

Chlorhexidine                 10 or 20cc syringe                     Vacuum Bottles

Angio-cath tubing- “custom angiographic kit by NAMIC”                

Lavender top, port-a-cult vial, red top, green top

In general, diagnostic (and many therapeutic) paracentesis fluid should be sent for:

(1) cell count and differential (green top (or lavender top), minimum 1 cc),

(2) gram stain and bacterial culture, as well as fungal and AFB when indicated (special green-top, agar-based aerobic port-a-cult culture bottle, or specimen cup, or regular blood culture bottles [blood culture bottles give the best yield]

(3) albumin (to calculate the serum-ascites albumin gradient, see SBP section below for interpretation) (red top, minimum 1 cc),

(4) consider total protein, LDH, amylase (if you suspect pancreatitis), cholesterol (if you suspect chylous ascites), depending on your diagnostic concern (red top).

(5) cytopath, depending on your diagnostic concern (any container: the more the better); add 5,000 U heparin/1 L bottle to prevent clotting.  If you are doing a procedure on the weekend, you can call the Pathology resident on call to pick up bottles for cytology, or you can place them in the refrigerator as soon as possible to preserve the cytology and deliver the bottles to cytology on Monday to the cytology department 4th floor.

 

Helpful Paracentesis Hints:  The lower quadrant approach is most often used.  Place the patient in a supine position.  Percuss to find the level of dullness.  Insert the needle along the anterior axillary line, lateral to the rectus sheath (halfway b/n the umbilicus and the anterior superior iliac spine) or 1-2 cm below the level of percussed dullness.

 

Thoracentesis

Single lumen central line kit OR Thoracentesis kit with turkel needle

Basic tray                     Chlorhexidine scrub       

Vacuum bottles (don’t need if using thoracentesis kit, it has a 2 liter bag in it)

Red top, lavender top, culture bottle

STAT CXR after procedure

 

In general, diagnostic +/- therapeutic thoracentesis fluid should be sent for:

(1) cell count and diff (green or lavender top),

(2) gram stain +/- culture (bacteriology, virology, mycology, AFB) (port-a-cult or specimen cup),

(3) glucose, protein*, LDH*, cholesterol (if you suspect chylous effusion) (red top),

(4) pH (green top) which should be walked to the critical care lab immediately,

(5) cytopath, depending on your diagnostic concern (in any container: the more the better); add 5,000 U heparin/1 L bottle to prevent clotting.

Look for specialized thoracentesis kits with nearly all the equipment needed.  Otherwise, the high-pressure angiographic tubing, with Kelly clamp and angiocath from the triple lumen kit is a good alternative.

 

*Send corresponding serum tests to calculate fluid to serum ratios.

 

Helpful Thoracentesis Hints:  View the CXR, so you know the area of interest.  Place the patient in a sitting position, leaning over a table.  Percuss out the effusion, noting the superior edge of dullness on the posterior chest wall.  Confirm with auscultation.  Insert the needle at the middle of the rib just below the superior edge of the dullness in the posterior axillary line.  Aim for the superior aspect of the rib (the neuro-vascular bundle runs under the rib).  Anesthetize the periosteum.  You will likely get pleural fluid while anesthetizing.  Exchange the needle for a larger one, and proceed with either a diagnostic or therapeutic tap.